Rapid identification of Clostridium difficile by toxin detection
نویسندگان
چکیده
منابع مشابه
Clostridium Difficile Toxin A+B Rapid Test
Clostridium Difficile Toxin A+B Rapid Test provides a procedure for a qualitative detection of Clostridium difficile toxin A (TcdA) and toxin B (TcdB) in two separate bands. A positive band of either toxin is a sign of an underlying Clostridium difficile infection (CDI), which should attract the attention of the physician. Samples used are liquid or semi-liquid human stool samples. Care must be...
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The binary toxin Clostridium difficile transferase (CDT) is frequently observed in C. difficile strains and is associated with an increased severity of C. difficile infection. CDT-producing C. difficile infections cause higher fatality rates than infections with CDT negative isolates. Thus, the rapid and accurate identification of a CDT positive C. difficile infection is critical for effective ...
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AIMS A set of five tests were developed and tested for their ability to confirm the identity of C difficile colonies within 30 minutes. METHODS The relevant substrates were incorporated into four filter paper squares attached to a plastic carrier (Diffstrip), five enzymes/products (prolyl aminopeptidase, galactosidase, leucine aminopeptidase, acid phosphatase and indole). The strips were inoc...
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Neutralization of Clostridium difficile toxin by Clostridium sordellii antitoxin was studied by cytotoxicity assay in tissue culture. The sources of toxin were stools from two patients with pseudomembranous colitis and a culture filtrate of C. difficile isolated from one of the patients. C. sordellii antitoxin was available either in monovalent form or as gas gangrene polyvalent antitoxin. The ...
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A modification of a selective medium for Clostridium difficile is described. The ability of Cl difficile to produce p-cresol from p-hydroxy phenyl acetic acid provides a means for the rapid, sensitive detection and presumptive identification of this species in faecal cultures.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1982
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.15.3.465-467.1982